By W.A. Wood (Eds.)
The severely acclaimed laboratory average, Methods in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. given that 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless proper this day - really an important booklet for researchers in all fields of lifestyles sciences
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54, 484 (1973). See Vol. 3 [12] and Vol. 8 [3]. 32 ANALrTICAL METHODS [6] [6] Estimation of 3-Deoxy-2-ketoaldonic Acids B y RICHARDCHABY,DANIELCHARON,ROBERTS. SARFATI, LADISLAS SZAB(~,and FRANCOISTRIGALO Estimation with Thiobarbituric Acid 1 Principle. Treatment of 3-deoxy-2-ketoaldonic acids with periodate yields 2,4-dioxobutyrate which condenses with 2-thiobarbituric acid to give a red dye 2 having an absorption maximum at 549 nm. 1 N N a 0 H . 1 N HC1 or N a 0 H , as required, and the volume is completed to 100 ml by addition of water.
3. The total pentose phosphate in a sample may be determined by omitting ribose 5-phosphate from the reaction mixture and adding ribose5-phosphate ketol isomerase, epimerase, and transketolase together. Under these conditions 2 molecules of pentose phosphate gives rise to the oxidation of 1 molecule of NADH. [9] Enzymic Determination of Lactic Acid By JOHANNES EVERSE Several methods have been described for the chemical determination of lactic acid concentrations in tissue extracts, sera, and other biological [9] ENZYMIC DETERMINATION OF LACTIC ACID 41 TRANSKETOLASE A340 1 -~i M I ERASE TIME FIG.
44, 254 (1971). 1 M substrate. 1 ml of 20% K O H . 5 ttmole of oxygen per micromole of substrate is consumed. 1 M LiC1 final). 6 ml of chloroform. The precipitate is removed b y centrifugation for 5 min at 3000 g. The decomposition products of phenazine are removed in the chloroform layer. Polarography o] the Enzymic Reaction Products. The polarograms of the oxidized substrates can be classified in three types. The first type belongs to the 3-uloses, produced in high yield, from lactulose (85%), lactose (80%), lactobionic acid (75%), methyl-fl-D-thiogalactose (72%), and melibiose (65%).
